After the death of Bishop Chacim, the Portuguese authorities started to bear hatred against the Church.  In 1834 the government ordered the evacuation of all the missionaries from Macao, and confiscated all the Church's possession causing a vacant bishopric administration for thirteen years.  In 1841, the Vatican appointed Nicolau Rodrigues Pereira de Borja to be the Bishop of Macao.  In fact, Bishop Borja had already been in Macao in 1802 and had acted as the rector of St. Joseph's Seminary.  In 1845 he died during his term of service after being the Bishop of Macao for four years.